citrate buffer (Thermo Fisher)

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Thermo Fisher citrate buffer
Citrate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/citrate+buffer+solution/Buffer+solution+pH+4%2C+Citrate+buffer/pm42353256-172-2-6
Average 96 stars, based on 1 article reviews

citrate buffer - by Bioz Stars, 2026-10

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Article Title: Paclitaxel resistance-associated metabolic events in ovarian cancer cells.
Article Snippet: 1 Associate Laboratory i4HB – Institute for Health and Bioeconomy, University of Porto, 4050-313 Porto, Portugal 2 UCIBIO – Applied Molecular Biosciences Unit, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal 3 Applied Molecular Biosciences Unit (UCIBIO), Toxicologic Pathology Research Laboratory, University Institute of Health Sciences (1H-TOXRUN, IUCS-CESPU), 4585-116 Gandra, Portugal 4 Associate Laboratory i4HB, Institute for Health and Bioeconomy, University Institute of Health Sciences (IUCS), University Polytechnic Higher Education Cooperative (CESPU), CRL, 4585-116 Gandra, Portugal Abstract Introduction The standard first-line management of advanced ovarian cancer (OC) comprises cytoreductive (debulking) surgery followed by platinum-based chemotherapy, most commonly a combination of carboplatin and paclitaxel (PTX).. However, the development of resistance to PTX frequently compromises treatment efficacy, resulting in disease recurrence and poorer clinical outcomes.. Although the metabolic mechanisms underlying carboplatin resistance have been extensively characterised, the specific metabolic alterations contributing to PTX resistance remain poorly understood.

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Article Title: Immuno-Golgi Method - Integrating Immunohistochemistry and Golgi Staining to Characterize Newborn Neurons Morphology
Article Snippet: .. − For Golgi-Cox solution: potassium dichromate (Sigma Aldrich, 207802); mercuric chloride (Sigma Aldrich, 215465); potassium chromate (Sigma Aldrich, 529508) − Kodak Fix solution (Rapid fixer; Sigma Aldrich) − For sucrose 6 % and 30 % solutions: sucrose (LabChem, 660112) − Ammonium hydroxide solution (Sigma Aldrich, 221228) − For chloroform-xylene-alcohol (CXA) solution (1 total – keep under fume hood): chloroform (Carlo Erba, 438601); xylene (Carlo Erba, 492306); alcohol (Carlo Erba, 4146312) − Sterile distilled water − For phosphate buffered saline 1X (PBS) solution: sodium chloride (Sigma Aldrich, S9888); potassium chloride (Sigma Aldrich, P3911); disodium hydrogen phosphate (Sigma Aldrich, S9763); potassium dihydrogen phosphate (Sigma Aldrich, P0662) − For PBS Tween 20 0.5 % (PBST) solution: tween 20 (Sigma Aldrich, P1379) − For Triton®-X 100 0.5 % solution: triton X100 (Sigma Aldrich, T8787) − For citrate buffer solution 10 mM: citric acid (Sigma Aldrich, C0759) − Saline 0.9 % − Anti-BrdU antibody (mouse anti-rat, Dako, M0744) − Anti-mouse Alexa Fluor 488 (Invitrogen, A32723) − DAPI (Invitrogen, D3571) − Normal goat serum (NGS, Sigma Aldrich, NS02L) − Eutasil (Ceva Saúde Animal, Portugal) − VECTASHIELD® Mounting Medium (Vector Labs) .. − 6-well and 24-well multiwell plate (Nunc) − 50 ml Falcon tubes − Plastic Pasteur pipettes − Scissors − Razor blade − Paint brush − Glass Bottles − SuperFrost® slides (Menzel-Gläser) − Coverslips − Cyanoacrylate

Sterility:

Saline:

Next-Generation Sequencing:

Blocking Assay:

Article Title: α-ketoglutarate/succinate ratio imbalance impairs thymine DNA glycosylase function and base excision repair process increasing susceptibility to pancreatic cancer.
Article Snippet: .. Antigen-retrieval was performed in the microwave in citrate buffer solution pH 6.0 (ScyTech) for 15 min. After blocking, sections were incubated overnight at 4°C with following primary antibodies: anti-Ecad (Invitrogen); anti-αSMA (Invitrogen); anti5-formylcytosine (Active Motif). .. After washing, cells were incubated for 1h at room temperature with a fluorescent secondary antibody (Alexa Fluor 488- or 546-conjugated, Life Technologies) and nuclei were counterstained with DRAQ5 (BioStatus).

Article Title: α-ketoglutarate/succinate ratio imbalance impairs thymine DNA glycosylase function and base excision repair process increasing susceptibility to pancreatic cancer
Article Snippet: .. Antigen-retrieval was performed in the microwave in citrate buffer solution pH 6.0 (ScyTech) for 15 min. After blocking, sections were incubated overnight at 4 °C with following primary antibodies: anti-Ecad (Invitrogen); anti-αSMA (Invitrogen); anti-5-formylcytosine (Active Motif). .. After washing, cells were incubated for 1 h at room temperature with a fluorescent secondary antibody (Alexa Fluor 488- or 546-conjugated, Life Technologies) and nuclei were counterstained with DRAQ5 (BioStatus).

Article Title: Cellular Anti-Apoptotic Effects of Dapagliflozin in Methotrexate-Induced Liver Toxicity: Bax/Bcl-2/Cyt-C/Cas-9/Cas-3 Signaling Pathway
Article Snippet: .. After 5 μ thick sections were taken from the paraffin blocks, they were deparaffinized and passed through alcohol series and boiled in citrate buffer solution for 20 min. https://doi.org/10.3390/ijms27052110 After 5 min treatment with H2O2 (TA-060-HP, Lab Vision Corporation, Fremont, CA, USA) and Ultra V Block (TA-125-UB, Thermo Scientific, Cheshire, UK) solution, they were incubated with Caspase-3 (sc-65497, Santa Cruz, CA, USA, 1:200), VEGF (sc-7269, Santa Cruz, CA, USA, 1:200), TNF alpha (sc-52746, Santa Cruz, CA, USA, 1:200), NFκB p65 (sc-8008, Santa Cruz, CA, USA, 1:200), and IL-1β (sc-52012, Santa Cruz, CA, USA, 1:200) for 60 min. Then they were incubated with secondary antibody (Biotinylated Goat AntiPolyvalent TP-125-BN, Thermo Scientific, Cheshire, UK) and Strepavidin HRP (Horse radish peroxidase) (TS-125-HR, Thermo Scientific, Cheshire, UK) for 30 min at room temperature. .. After DAB (3,3′-diaminobenzidine) (TA-125 HD, Thermo Scientific, Cheshire, UK) solution, tissues were counter-stained with Mayer’s Hematoxylin and covered with Entellan.

Article Title: Cellular Anti-Apoptotic Effects of Dapagliflozin in Methotrexate-Induced Liver Toxicity: Bax/Bcl-2/Cyt-C/Cas-9/Cas-3 Signaling Pathway
Article Snippet: .. After 5 μ thick sections were taken from the paraffin blocks, they were deparaffinized and passed through alcohol series and boiled in citrate buffer solution for 20 min. After 5 min treatment with H 2 O 2 (TA-060-HP, Lab Vision Corporation, Fremont, CA, USA) and Ultra V Block (TA-125-UB, Thermo Scientific, Cheshire, UK) solution, they were incubated with Caspase-3 (sc-65497, Santa Cruz, CA, USA, 1:200), VEGF (sc-7269, Santa Cruz, CA, USA, 1:200), TNF alpha (sc-52746, Santa Cruz, CA, USA, 1:200), NFκB p65 (sc-8008, Santa Cruz, CA, USA, 1:200), and IL-1β (sc-52012, Santa Cruz, CA, USA, 1:200) for 60 min. Then they were incubated with secondary antibody (Biotinylated Goat Anti-Polyvalent TP-125-BN, Thermo Scientific, Cheshire, UK) and Strepavidin HRP (Horse radish peroxidase) (TS-125-HR, Thermo Scientific, Cheshire, UK) for 30 min at room temperature. .. After DAB (3,3′-diaminobenzidine) (TA-125 HD, Thermo Scientific, Cheshire, UK) solution, tissues were counter-stained with Mayer’s Hematoxylin and covered with Entellan.

Incubation:

Article Title: α-ketoglutarate/succinate ratio imbalance impairs thymine DNA glycosylase function and base excision repair process increasing susceptibility to pancreatic cancer
Article Snippet: .. Antigen-retrieval was performed in the microwave in citrate buffer solution pH 6.0 (ScyTech) for 15 min. After blocking, sections were incubated overnight at 4 °C with following primary antibodies: anti-Ecad (Invitrogen); anti-αSMA (Invitrogen); anti-5-formylcytosine (Active Motif). .. After washing, cells were incubated for 1 h at room temperature with a fluorescent secondary antibody (Alexa Fluor 488- or 546-conjugated, Life Technologies) and nuclei were counterstained with DRAQ5 (BioStatus).

Article Title: Cellular Anti-Apoptotic Effects of Dapagliflozin in Methotrexate-Induced Liver Toxicity: Bax/Bcl-2/Cyt-C/Cas-9/Cas-3 Signaling Pathway
Article Snippet: .. After 5 μ thick sections were taken from the paraffin blocks, they were deparaffinized and passed through alcohol series and boiled in citrate buffer solution for 20 min. After 5 min treatment with H 2 O 2 (TA-060-HP, Lab Vision Corporation, Fremont, CA, USA) and Ultra V Block (TA-125-UB, Thermo Scientific, Cheshire, UK) solution, they were incubated with Caspase-3 (sc-65497, Santa Cruz, CA, USA, 1:200), VEGF (sc-7269, Santa Cruz, CA, USA, 1:200), TNF alpha (sc-52746, Santa Cruz, CA, USA, 1:200), NFκB p65 (sc-8008, Santa Cruz, CA, USA, 1:200), and IL-1β (sc-52012, Santa Cruz, CA, USA, 1:200) for 60 min. Then they were incubated with secondary antibody (Biotinylated Goat Anti-Polyvalent TP-125-BN, Thermo Scientific, Cheshire, UK) and Strepavidin HRP (Horse radish peroxidase) (TS-125-HR, Thermo Scientific, Cheshire, UK) for 30 min at room temperature. .. After DAB (3,3′-diaminobenzidine) (TA-125 HD, Thermo Scientific, Cheshire, UK) solution, tissues were counter-stained with Mayer’s Hematoxylin and covered with Entellan.

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